Abstract The aim of this study was to evaluate the oxidative stress in ovaries and corpus luteum (CL) of Bos taurus indicus females and the oxidant effect of CL in ovarian tissues in regions near, intermediate, or distant from it. Ovaries (n=12) of Nelore heifers (n=6) were collected from a slaughterhouse and fragmented. Experiment 1, each ovary was obtained from three fragments, resulting in 18 fragments of ovaries with CL (OV+CL) and another 18 fragments of ovaries without CL (OV-CL). Three fragments were generated from CL, totaling 18 CL fragments. In experiment 2, the ovarian fragments were removed from specific regions near, intermediate, or distant from the CL. All the fragments were placed in Eppendorf-type microtubes (1 mL), kept in a thermal container at 4 ºC, and then stored in a -80 ºC freezer for analysis of oxidative stress (TBARS and NBT) and antioxidant potential (FRAP and ABTS). In the antioxidant activity analysis, luteal tissues showed more antioxidant activity than ovarian tissue (FRAP = P < 0.0001; ABTS = P < 0.02). In the oxidative stress analysis, CL had lower levels of reactive oxygen species (ROS; TBARS = P < 0.03; NBT = P < 0.0001) than ovarian tissues. There was no difference in antioxidant activity and oxidative stress between the fragments obtained from different regions (OV+CL versus OV-CL; P > 0.05). The presence of CL in the ovaries of Bos taurus indicus females did not influence the oxidative stress or antioxidant potential of the gonad. Thus, the removal of ovarian fragments with or without the presence of CL indicates that biotechnologies such as in vitro follicle cultivation is possible.

RESUMO Este estudo revisou sistematicamente a literatura sobre ‘necessidades em(de) saúde’. Tratou-se de uma revisão integrativa utilizando o portal Biblioteca Virtual em Saúde. Português, espanhol e inglês foram os idiomas utilizados e recuperaram-se apenas os textos completos e com foco específico no Sistema Único de Saúde. Totalizaram-se 17 artigos incluídos, dos quais 88,8% estavam indexados na base Literatura Latino-Americana e do Caribe em Ciências da Saúde. Há uma variada forma de apropriação das ‘necessidades em(de) saúde’, termo cuja definição não é bem delimitada pelos autores, direcionando- o a três núcleos de sentido: ‘direito social constituído’, ‘conjunto articulado da efetividade dos direitos sociais’ e ‘ajuste entre as condições de vida e trabalho com a diversidade inerente às coletividades’. Diversas implicações para o Sistema Único de Saúde foram apresentadas e puderam ser compiladas em sete blocos: ‘capitalismo e sua crise’, ‘financiamento’, ‘características da formação social brasileira’, ‘problemas da gestão pública na saúde’, ‘mescla público-privado’, ‘problemas terminológicos’ e ‘escassez de recursos’. Por fim, os principais desafios da aplicação das necessidades no Sistema Único de Saúde transitam entre o simbólico, o crítico-político, a coerência entre financiamento-princípios, o Estado, a política macroeconômica, a gestão e as ações locais. Investir na superação dos desafios elencados pode ser um guia para a efetivação das necessidades como o centro da ação sanitária.

ABSTRACT This study reviewed systematically the literature on ‘needs in health/health needs’. This was an integrative review using the Virtual Health Library portal. Portuguese, English and Spanish were the languages used and only the full texts were recovered with a specific focus on the Unified Health System. A total of 17 articles were included, of which 88.8% were indexed in the Latin American and Caribbean Health Sciences database. There is a varied form of appropriation of the theme ‘needs in health/health needs’, a very broad concept whose definition is not well delimited by the authors, directing it to three nuclei of meaning: ‘constituted social right’, ‘articulated set of the effectiveness of social rights’ and ‘adjustment between living and working conditions with the inherent diversity of collectivities’. Several implications for the Unified Health System were presented and could be compiled in seven blocks: ‘capitalism and its crisis’, ‘financing’, ‘characteristics of the Brazilian social formation’, ‘problems of public health management’, ‘public-private mix’, ‘terminological problems’ and ‘scarcity of resources’. Finally, the main challenges of applying the needs in the Unified Health System move between the symbolic, the critical-political, the coherence between financing-principles, the State, macroeconomic policy, management and local actions. Investing in overcoming the challenges listed can be a guide in realizing needs as the center of health action.

RESUMO Este estudo revisou sistematicamente a literatura sobre ‘necessidades em(de) saúde’. Tratou-se de uma revisão integrativa utilizando o portal Biblioteca Virtual em Saúde. Português, espanhol e inglês foram os idiomas utilizados e recuperaram-se apenas os textos completos e com foco específico no Sistema Único de Saúde. Totalizaram-se 17 artigos incluídos, dos quais 88,8% estavam indexados na base Literatura Latino-Americana e do Caribe em Ciências da Saúde. Há uma variada forma de apropriação das ‘necessidades em(de) saúde’, termo cuja definição não é bem delimitada pelos autores, direcionando- o a três núcleos de sentido: ‘direito social constituído’, ‘conjunto articulado da efetividade dos direitos sociais’ e ‘ajuste entre as condições de vida e trabalho com a diversidade inerente às coletividades’. Diversas implicações para o Sistema Único de Saúde foram apresentadas e puderam ser compiladas em sete blocos: ‘capitalismo e sua crise’, ‘financiamento’, ‘características da formação social brasileira’, ‘problemas da gestão pública na saúde’, ‘mescla público-privado’, ‘problemas terminológicos’ e ‘escassez de recursos’. Por fim, os principais desafios da aplicação das necessidades no Sistema Único de Saúde transitam entre o simbólico, o crítico-político, a coerência entre financiamento-princípios, o Estado, a política macroeconômica, a gestão e as ações locais. Investir na superação dos desafios elencados pode ser um guia para a efetivação das necessidades como o centro da ação sanitária.

ABSTRACT This study reviewed systematically the literature on ‘needs in health/health needs’. This was an integrative review using the Virtual Health Library portal. Portuguese, English and Spanish were the languages used and only the full texts were recovered with a specific focus on the Unified Health System. A total of 17 articles were included, of which 88.8% were indexed in the Latin American and Caribbean Health Sciences database. There is a varied form of appropriation of the theme ‘needs in health/health needs’, a very broad concept whose definition is not well delimited by the authors, directing it to three nuclei of meaning: ‘constituted social right’, ‘articulated set of the effectiveness of social rights’ and ‘adjustment between living and working conditions with the inherent diversity of collectivities’. Several implications for the Unified Health System were presented and could be compiled in seven blocks: ‘capitalism and its crisis’, ‘financing’, ‘characteristics of the Brazilian social formation’, ‘problems of public health management’, ‘public-private mix’, ‘terminological problems’ and ‘scarcity of resources’. Finally, the main challenges of applying the needs in the Unified Health System move between the symbolic, the critical-political, the coherence between financing-principles, the State, macroeconomic policy, management and local actions. Investing in overcoming the challenges listed can be a guide in realizing needs as the center of health action.

Abstract Background: The Val66Met polymorphism of the brain-derived neurotrophic factor (BDNF) gene is a potential biomarker of vulnerability to pain. Thus, the present study aimed to investigate the association of this polymorphism with clinical and biopsychosocial factors in patients with chronic low back pain (CLBP). Methods: A total of 107 individuals with CLBP answered questionnaires that were validated and adapted for the Brazilian population, including the Brief Inventory of Pain, the Central Sensitization Inventory, the Roland Morris Disability Questionnaire, the Tampa Scale for Kinesiophobia, the Pain Catastrophizing Scale, the Survey of Pain Attitude-Brief, and the Hospital Anxiety and Depression Scale. All of the subjects were genotyped for the BDNF Val66Met polymorphism. Results: The sample showed moderate scores of disability, central sensitization, and kinesiophobia, in addition to mild anxiety, hopelessness, and ruminant thoughts. No significant association was observed between the Val66Met polymorphism and the variables analyzed. Besides, there was no relationship between the BDNF Val66Met polymorphism with CSI, catastrophization, or disabilities that were generated by CLBP. Conclusions: The results showed that the Val66Met polymorphism of the BDNF gene was not associated with clinical and biopsychosocial characteristics of CLBP in the sample studied.

OBJETIVO: avaliar, através de fotografias clínicas, entre estudantes de Odontologia e ortodontistas, o grau de influência que ligaduras elásticas estéticas pigmentadas exercem sobre a atratividade do sorriso. MÉTODOS: foram utilizadas 16 fotografias clínicas faciais do sorriso e 16 de sorriso aproximado de um único paciente portando braquetes ortodônticos de porcelana monocristalina, fio de NiTi teflonado e ligaduras elásticas estéticas de cinco marcas comerciais diferentes, distribuídas em oito grupos, G1 a G8 (Morelli, Ortho Technology, TP Orthodontics, 3M/Unitek clear, 3M/Unitek obscure, American Orthodontics clear, American Orthodontics pearl e American Orthodontics metalic pearl). Foram utilizadas 20 ligaduras de cada grupo, totalizando 160 ligaduras. Metade delas foi utilizada em estado natural e a outra metade após pigmentação in vitro. Todas as fotografias foram julgadas por 40 avaliadores, sendo 20 ortodontistas e 20 estudantes de Odontologia. RESULTADOS: para ortodontistas, as ligaduras American Orthodontics pearl (G7) foram as que menos influenciaram o grau de atratividade do sorriso nos dois tipos de fotografias utilizadas. Para os estudantes de Odontologia, nas fotografias faciais do sorriso, as que obtiveram o melhor desempenho foram Morelli (G1), American Orthodontics clear (G6) e American Orthodontics pearl (G7) e, nas fotografias de sorriso aproximado, American Orthodontics pearl, metalic pearl e clear (G7, G8 e G6, respectivamente). CONCLUSÕES: tanto para ortodontistas quanto para estudantes de Odontologia, a pigmentação das ligaduras elásticas influenciou de forma negativa o grau de atratividade dos sorrisos nos dois tipos de fotografias clínicas avaliadas.

OBJECTIVE: To evaluate the perception of dental students and orthodontists on the degree of influence that pigmented esthetic elastic ligatures have on smile attractiveness, by judging clinical photographs. METHODS: Sixteen clinical facial photographs of the smile and 16 close up images of the smile of a single patient wearing monocrystalline porcelain orthodontic brackets, Teflon coated NiTi wire brackets and esthetic elastic ligatures of five different commercial brands were distributed into eight groups, G1 to G8 (Morelli®, Ortho Tecnology™, TP Orthodontics™, Unitek/3M™clear, Unitek/3M™ obscure, American Orthodontics™ clear, American Orthodontics™ pearl and American Orthodontics™ metallic pearl). Twenty ligatures were used in each group, totaling 160 ligatures. Half of them were used in their natural state, and the other half after in vitro pigmentation. All the photographs were judged by 40 evaluators, 20 orthodontists and 20 dental students. RESULTS: For orthodontists, American™ pearl (G7) ligatures were those that least influenced the degree of attractiveness of the smile in the two types of photographs used. For the dental students, in the facial photographs of the smile, ligatures with the best performance were Morelli® (G1), American™ clear (G6) and American™ pearl (G7) and in the close up photographs of the smile, American™ pearl, metallic pearl and clear (G7, G8 and G6). CONCLUSIONS: For both orthodontists and dental students, pigmentation of the elastic ligatures had a negative influence on the degree of attractiveness of smiles in the two types of clinical photographs evaluated.

Ring Opening Metathesis Polymerization (ROMP) of cyclic olefins is a powerful transition metal-catalyzed reaction for syntheses of polymers and copolymers. The key feature of this reaction is the [2+2]-cycloaddition mechanism, with retention of the olefinic unsaturation in the polymer chain and occurrence of living polymerization. With the development of metal-carbene type catalysts for this process, many addressed polymeric materials have been successfully prepared to be employed in several fields of the science and technology. This review summarizes recent examples of syntheses of polymers with amphiphilic features such as block, graft, brush or star copolymers; as well syntheses of biomaterials, dendronized architectures, photoactive polymers, cross-linked or self-healing materials, and polymers from renewed supplies.

OBJETIVO: O uso de terapia medicamentosa, como cabergolina, octreotide e octreotide de longa duração, tem apresentado resultados variados no tratamento da produção excessiva de hormônio de crescimento (GH) em pacientes com síndrome de McCune-Albright. MÉTODOS: Foi relatado o caso de uma paciente de 29 anos apresentando síndrome de McCune-Albright com queixas de crescimento ósseo excessivo. RESULTADOS: A paciente apresentava adenoma pituitário com envolvimento da artéria carótida interna direita e produção excessiva de GH (sem supressão de GH após o teste de supressão com glicose). Por causa do aumento importante da espessura dos ossos da base do crânio, a abordagem cirúrgica foi considerada pouco efetiva e a paciente foi submetida à terapia medicamentosa com octreotide de longa duração e cabergolina. No seguimento de um ano, os níveis de GH e IGF-1 estavam normais e os efeitos adversos não eram presentes. CONCLUSÃO: A terapia medicamentosa fundamentada na associação de cabergolina e octreotide é segura e capaz de alcançar controle hormonal completo no tratamento de acromegalia na síndrome de McCune-Albright.

OBJECTIVES: The use of drug therapy based on cabergoline, octreotide and long-acting release (LAR) octreotide has presented varying results in the treatment of GH excessive production in patients with McCune-Albright Syndrome. METHODS: We report the case of a 29 year-old female patient presenting McCune-Albright Syndrome and complaint of excessive bone growth. RESULTS: The patient presented a pituitary adenoma involving the right internal carotid artery and excessive secretion of growth hormone (no GH suppression was observed after the oral glucose tolerance test). Due to the presence of diffuse thickness in skull base bones, surgical approach was not considered effective and the patient was submitted to drug therapy with octreotide LAR and cabergoline. At the one year follow-up, GH and IGF-1 levels were normal and no adverse effects were present. CONCLUSION: The use of drug therapy based on the association of cabergoline and octreotide is safe and able to achieve complete hormonal control in the treatment of acromegaly for McCune-Albright patients.